Fire crews were called to the Good Things Brewing Company Ltd in Sandhill Lane off the Eridge Road near Crowborough on Sunday (July 25).
East Sussex Fire & Rescue Service said the blaze broke out around 1pm and six fire engines and a control unit were sent to the scene.
Local people were asked to keep windows and doors closed as firefighters battled the blaze, which gutted the barn.
The Good Things Brewing Company said they have now started a fundraiser to rebuild the barn.
In a statement on their Instagram page, the brewery’s Chris Drummond said: “Our beloved 17th-century barn, the home to our brewery, was struck by lightning today and has sadly burned to the ground. Thankfully the team and I are all safe and no one was hurt.
“The barn is something our family and brew team treasured, with so many fond memories, and we are deeply saddened but we are very thankful no one was on site today.
“We will be back in touch over the next few days, thank you to everyone who has offered their support so far.”
They have set up an online GoFundMe page to start the clearing and reconstruction of the site. At the time of going to press, the fundraiser had received £10,550 in donations.
To donate to help rebuild the Good Things Brewery, click on this link: gofundme.com/f/help-rebuild-our-brewery-home